• Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
  • Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PKD2-Ser812 antibody (778-827 aa) (STJ91356)

SKU:
STJ91356

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Polycystin-2-Ser812 (778-827 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PKD2
Gene ID: 5311
Uniprot ID: PKD2_HUMAN
Immunogen Region: 778-827 aa
Specificity: Phospho-PKD2 (S812) Polyclonal Antibody detects endogenous levels of PKD2S812.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PKD2 around the phosphorylation site of Ser812 at the amino acid range 778-827
Post Translational Modifications Phosphorylated. Phosphorylation is important for protein function.a mutant that lacks the N-terminal phosphorylation sites cannot complement a zebrafish pkd2-deficient mutant. PKD-mediated phosphorylation at the C-terminus regulates its function in the release of Ca(2+) stores from the endoplasmic reticulum. PKA-mediated phosphorylation at a C-terminal site strongly increases the open probability of the channel, but does not increase single channel conductance. N-glycosylated. The four subunits in a tetramer probably differ in the extent of glycosylation.simultaneous glycosylation of all experimentally validated sites would probably create steric hindrance. Thus, glycosylation at Asn-305 is not compatible with glycosylation at Asn-328.only one of these two residues is glycosylated at a given time.
Function Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B. Can also form a functional, homotetrameric ion channel. Functions as a cation channel involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. Functions as outward-rectifying K(+) channel, but is also permeable to Ca(2+), and to a much lesser degree also to Na(+). May contribute to the release of Ca(2+) stores from the endoplasmic reticulum. Together with TRPV4, forms mechano- and thermosensitive channels in cilium. PKD1 and PKD2 may function through a common signaling pathway that is necessary to maintain the normal, differentiated state of renal tubule cells. Acts as a regulator of cilium length, together with PKD1. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. Also involved in left-right axis specification via its role in sensing nodal flow.forms a complex with PKD1L1 in cilia to facilitate flow detection in left-right patterning. Detection of asymmetric nodal flow gives rise to a Ca(2+) signal that is required for normal, asymmetric expression of genes involved in the specification of body left-right laterality.
Protein Name Polycystin-2
Pc2
Autosomal Dominant Polycystic Kidney Disease Type Ii Protein
Polycystic Kidney Disease 2 Protein
Polycystwin
R48321
Transient Receptor Potential Cation Channel Subfamily P Member 2
Database Links Reactome: R-HSA-5620916
Cellular Localisation Cell Projection
Cilium Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Cell Membrane
Basolateral Cell Membrane
Cytoplasmic Vesicle Membrane
Golgi Apparatus
Pkd2 Localization To The Plasma And Ciliary Membranes Requires Pkd1
Pkd1:Pkd2 Interaction Is Required To Reach The Golgi Apparatus Form Endoplasmic Reticulum And Then Traffic To The Cilia
Retained In The Endoplasmic Reticulum By Interaction With Pacs1 And Pacs2
Detected On Kidney Tubule Basolateral Membranes And Basal Cytoplasmic Vesicles
Cell Surface And Cilium Localization Requires Ganab
Alternative Antibody Names Anti-Polycystin-2 antibody
Anti-Pc2 antibody
Anti-Autosomal Dominant Polycystic Kidney Disease Type Ii Protein antibody
Anti-Polycystic Kidney Disease 2 Protein antibody
Anti-Polycystwin antibody
Anti-R48321 antibody
Anti-Transient Receptor Potential Cation Channel Subfamily P Member 2 antibody
Anti-PKD2 antibody
Anti-TRPP2 antibody

Information sourced from Uniprot.org

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