• Western blot analysis of lysates from wild type (WT) 、 293F cells treated by PMA、293F cells treated by PMA after transfected with PRKCA and 293F cells treated by PMA after transfected with PRKCD using Phospho-PKC alpha-T410+PKC delta-T410 Rabbit monoclonal antibody (STJ11105905) at 1:2000 dilution. 293F、293F-PRKCA-Transfected and 293F-PRKCD-Transfected cells were treated by PMA (200 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-PKC alpha+PKC delta-T410-T410 antibody [S5905RM] (STJ11105905)

SKU:
STJ11105905

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5905RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:3000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKCD
PRKCA
Gene ID: 5580
5578
Uniprot ID: KPCD_HUMAN
KPCA_HUMAN
Specificity: A synthetic phosphorylated peptide around T410 of human PRKCZ (NP_002735.3).
Immunogen Sequence: TTSTFC
Tissue Specificity
Alternative Antibody Names Anti-Protein Kinase C Delta Type antibody
Anti-Tyrosine-Protein Kinase Prkcd antibody
Anti-Npkc-Delta Cleaved Into - Protein Kinase C Delta Type Regulatory Subunit - Protein Kinase C Delta Type Catalytic Subunit antibody
Anti-Sphingosine-Dependent Protein Kinase-1 antibody
Anti-Sdk1 antibody
Anti-PRKCD antibody
Anti-PKCD antibody
Anti-Protein Kinase C Alpha Type antibody
Anti-Pkc-A antibody
Anti-Pkc-Alpha antibody
Anti-PRKCA antibody
Anti-PKCA antibody
Anti-PRKACA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance