Western blot analysis of various lysates using Phospho-PEA15-S104 Rabbit pAb (STJ11101012) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 ยฐC for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-PEA15-S104 antibody (STJ11101012)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11101012
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General Information

Short DescriptionRabbit polyclonal Phospho-PEA15-S104 antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPEA15
Gene ID8682
Uniprot IDPEA15_HUMAN
Immunogen SequenceIPSAK
SpecificityA synthetic phosphorylated peptide around S104 of human PEA15 (NP_003759.1).

Additional Info

Tissue Specificity Ubiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.
Post Translational Modifications Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.
Function Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm. Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
Protein Name Astrocytic Phosphoprotein Pea-15
15 Kda Phosphoprotein Enriched In Astrocytes
Phosphoprotein Enriched In Diabetes
Ped
Database Links Reactome: R-HSA-112409
Reactome: R-HSA-5673001
Cellular Localisation Cytoplasm
Associated With Microtubules
Alternative Antibody Names Anti-Astrocytic Phosphoprotein Pea-15 antibody
Anti-15 Kda Phosphoprotein Enriched In Astrocytes antibody
Anti-Phosphoprotein Enriched In Diabetes antibody
Anti-Ped antibody
Anti-PEA15 antibody

Information sourced from Uniprot.org

Citations

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