| Host: |
Rabbit |
| Applications: |
WB/ELISA/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Pyruvate dehydrogenase kinase isozyme 1, mitochondrial-Tyr243 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-p 1:50-300ELISA 2000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PDK1 |
| Gene ID: |
5163 |
| Uniprot ID: |
PDK1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of Human, Mouse, Rat PDHK1 (Phospho Tyr243) |
| Immunogen: |
Synthesized peptide derived from human PDHK1 (Phospho Tyr243) |
| Tissue Specificity | Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas. |
| Post Translational Modifications | Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect. |
| Function | Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. |
| Protein Name | Pyruvate DehydrogenaseAcetyl-Transferring Kinase Isozyme 1 - MitochondrialPyruvate Dehydrogenase Kinase Isoform 1Pdh Kinase 1 |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase antibodyAnti-Acetyl-Transferring Kinase Isozyme 1 - Mitochondrial antibodyAnti-Pyruvate Dehydrogenase Kinase Isoform 1 antibodyAnti-Pdh Kinase 1 antibodyAnti-PDK1 antibodyAnti-PDHK1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
