Anti-Phospho-PDE4D PKA site antibody (50-100) {Biotin} (STJ502617)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationBiotin
IsotypeIgG
ImmunogenSynthetic peptide selective for PDE4D PKA site taken within amino acid region 50-100 on human PDE4D8 protein. Phosphorylated Serine 190 residue. Derived around the PKA phosphorylation site of serine 190: R-E-SP-F-L
General Information
| Short Description | Rabbit polyclonal antibody anti-Phospho-PDE4D PKA site (50-100) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IHC-P/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Biotin |
| Concentration | 0.57 ยตg/ยตl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500DB: 1:10, 000ELISA: 1:10, 000IP: 1:150IHC: 1:50-1:100 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20ยฐC for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | PDE4D |
| Gene ID | 5144 |
| Uniprot ID | PDE4D_HUMAN |
| Immunogen | Synthetic peptide selective for PDE4D PKA site taken within amino acid region 50-100 on human PDE4D8 protein. Phosphorylated Serine 190 residue. Derived around the PKA phosphorylation site of serine 190: R-E-SP-F-L |
| Immunogen Region | 50-100 |
| Specificity | Detects endogenous levels of PDE4D only when phosphorylated at serine 190. |
Additional Info
| Tissue Specificity | Expressed in colonic epithelial cells (at protein level). Widespread.most abundant in skeletal muscle. Isoform 6: Detected in brain. Isoform 8: Detected in brain, placenta, lung and kidney. Isoform 7: Detected in heart and skeletal muscle. |
| Post Translational Modifications | Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation. Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation. |
| Function | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
| Protein Name | 3' -5'-Cyclic-Amp Phosphodiesterase 4dDpde3Pde43Camp-Specific Phosphodiesterase 4d |
| Database Links | Reactome: R-HSA-180024Reactome: R-HSA-418555 |
| Cellular Localisation | Apical Cell MembraneCytoplasmMembraneCytoskeletonMicrotubule Organizing CenterCentrosomeFound In The Soluble FractionAssociated With MembranesAnd Associated With The Cytoskeleton And The CentrosomeColocalized With Shank2 To The Apical Membrane Of Colonic Crypt Cells |
| Alternative Antibody Names | Anti-3' -5'-Cyclic-Amp Phosphodiesterase 4d antibodyAnti-Dpde3 antibodyAnti-Pde43 antibodyAnti-Camp-Specific Phosphodiesterase 4d antibodyAnti-PDE4D antibodyAnti-DPDE3 antibody |
Information sourced from Uniprot.org