• Western blot analysis of lysates from rat heart, using PAK3 (Phospho-Ser154) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using PAK3 (Phospho-Ser154) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PAK3-Ser154 antibody (121-170 aa) (STJ90955)

SKU:
STJ90955

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase PAK 3-Ser154 (121-170 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAK3
Gene ID: 5063
Uniprot ID: PAK3_HUMAN
Immunogen Region: 121-170 aa
Specificity: Phospho-PAK Beta (S154) Polyclonal Antibody detects endogenous levels of PAK Beta protein only when phosphorylated at S154.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PAK3 around the phosphorylation site of Ser154 at the amino acid range 121-170
Post Translational Modifications Autophosphorylated when activated by CDC42/p21. Neddylated.
Function Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses.this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC).
Protein Name Serine/Threonine-Protein Kinase Pak 3
Beta-Pak
Oligophrenin-3
P21-Activated Kinase 3
Pak-3
Database Links Reactome: R-HSA-202433
Reactome: R-HSA-389359
Reactome: R-HSA-3928664
Reactome: R-HSA-399954
Reactome: R-HSA-428540
Reactome: R-HSA-5218920
Reactome: R-HSA-5621575
Reactome: R-HSA-5627123
Reactome: R-HSA-5687128
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013409
Reactome: R-HSA-9013420
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Pak 3 antibody
Anti-Beta-Pak antibody
Anti-Oligophrenin-3 antibody
Anti-P21-Activated Kinase 3 antibody
Anti-Pak-3 antibody
Anti-PAK3 antibody
Anti-OPHN3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance