| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-eNOS-S1177 is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NOS3 |
| Gene ID: |
4846 |
| Uniprot ID: |
NOS3_HUMAN |
| Immunogen: |
A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2). |
| Immunogen Sequence: |
TSRIRTQSFSLQERQ |
| Tissue Specificity | Platelets, placenta, liver and kidney. |
| Post Translational Modifications | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity. |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. |
| Protein Name | Nitric Oxide Synthase 3Constitutive NosCnosEc-NosNos Type IiiNosiiiNitric Oxide Synthase - EndothelialEndothelial NosEnos |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1474151Reactome: R-HSA-203615Reactome: R-HSA-203641Reactome: R-HSA-203754Reactome: R-HSA-392154Reactome: R-HSA-5218920Reactome: R-HSA-9009391 |
| Cellular Localisation | Cell MembraneMembraneCaveolaCytoplasmCytoskeletonGolgi ApparatusSpecifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell CycleWhich Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase 3 antibodyAnti-Constitutive Nos antibodyAnti-Cnos antibodyAnti-Ec-Nos antibodyAnti-Nos Type Iii antibodyAnti-Nosiii antibodyAnti-Nitric Oxide Synthase - Endothelial antibodyAnti-Endothelial Nos antibodyAnti-Enos antibodyAnti-NOS3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
