Anti-Phospho-NOS2-Tyr151 antibody (117-166 aa) (STJ91227)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human iNOS around the phosphorylation site of Tyr151 at the amino acid range 117-166
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Nitric oxide synthase, inducible-Tyr151 (117-166 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NOS2 |
| Gene ID | 4843 |
| Uniprot ID | NOS2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human iNOS around the phosphorylation site of Tyr151 at the amino acid range 117-166 |
| Immunogen Region | 117-166 aa |
| Specificity | Phospho-NOS2 (Y151) Polyclonal Antibody detects endogenous levels of NOS2 protein only when phosphorylated at Y151. |
Additional Info
| Post Translational Modifications | Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation. |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8. |
| Protein Name | Nitric Oxide Synthase - InducibleHepatocyte NosHep-NosInducible No SynthaseInducible NosInosNos Type IiPeptidyl-Cysteine S-Nitrosylase Nos2 |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-392154Reactome: R-HSA-6785807Reactome: R-HSA-9033241Reactome: R-HSA-9636249 |
| Cellular Localisation | CytoplasmCytosolLocalizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4Exhibits A More Diffuse Cytosolic Localization |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase - Inducible antibodyAnti-Hepatocyte Nos antibodyAnti-Hep-Nos antibodyAnti-Inducible No Synthase antibodyAnti-Inducible Nos antibodyAnti-Inos antibodyAnti-Nos Type Ii antibodyAnti-Peptidyl-Cysteine S-Nitrosylase Nos2 antibodyAnti-NOS2 antibodyAnti-NOS2A antibody |
Information sourced from Uniprot.org