| Host: |
Rabbit |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-iNOS-Tyr-1055 is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICC 1:400IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NOS2 |
| Gene ID: |
4843 |
| Uniprot ID: |
NOS2_HUMAN |
| Specificity: |
The antibody detects a 130 kDa* protein corresponding to induced NOS on SDS-PAGE immunoblots of mouse macrophages treated with LPS followed by pervanadate. The antibody also detects a 150 kDa doublet that corresponds with higher molecular weight form |
| Immunogen: |
Phospho-iNOS (Tyr-1055) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Tyr-1055 in human iNOS. This sequence is well conserved in rat and mouse iNOS, and has some homology to the conserved sites in eNOS (Tyr-108 |
| Post Translational Modifications | Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation. |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8. |
| Protein Name | Nitric Oxide Synthase - InducibleHepatocyte NosHep-NosInducible No SynthaseInducible NosInosNos Type IiPeptidyl-Cysteine S-Nitrosylase Nos2 |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-392154Reactome: R-HSA-6785807Reactome: R-HSA-9033241Reactome: R-HSA-9636249 |
| Cellular Localisation | CytoplasmCytosolLocalizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4Exhibits A More Diffuse Cytosolic Localization |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase - Inducible antibodyAnti-Hepatocyte Nos antibodyAnti-Hep-Nos antibodyAnti-Inducible No Synthase antibodyAnti-Inducible Nos antibodyAnti-Inos antibodyAnti-Nos Type Ii antibodyAnti-Peptidyl-Cysteine S-Nitrosylase Nos2 antibodyAnti-NOS2 antibodyAnti-NOS2A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
