Anti-Phospho-NFKBIB-Thr19 antibody (4-53 aa) (STJ90460)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human IkappaB-beta around the phosphorylation site of Thr19 at the amino acid range 4-53
General Information
| Short Description | Rabbit polyclonal anti-Phospho-NF-kappa-B inhibitor beta-Thr19 (4-53 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NFKBIB |
| Gene ID | 4793 |
| Uniprot ID | IKBB_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human IkappaB-beta around the phosphorylation site of Thr19 at the amino acid range 4-53 |
| Immunogen Region | 4-53 aa |
| Specificity | Phospho-I Kappa B-Beta (T19) Polyclonal Antibody detects endogenous levels of I Kappa B-Beta protein only when phosphorylated at T19. |
Additional Info
| Post Translational Modifications | Phosphorylated by RPS6KA1.followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation. |
| Function | Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation. |
| Protein Name | Nf-Kappa-B Inhibitor BetaNf-Kappa-BibI-Kappa-B-BetaIkb-BIkb-BetaIkappabbetaThyroid Receptor-Interacting Protein 9Tr-Interacting Protein 9Trip-9 |
| Database Links | Reactome: R-HSA-1169091Reactome: R-HSA-1810476Reactome: R-HSA-445989Reactome: R-HSA-933542 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Nf-Kappa-B Inhibitor Beta antibodyAnti-Nf-Kappa-Bib antibodyAnti-I-Kappa-B-Beta antibodyAnti-Ikb-B antibodyAnti-Ikb-Beta antibodyAnti-Ikappabbeta antibodyAnti-Thyroid Receptor-Interacting Protein 9 antibodyAnti-Tr-Interacting Protein 9 antibodyAnti-Trip-9 antibodyAnti-NFKBIB antibodyAnti-IKBB antibodyAnti-TRIP9 antibody |
Information sourced from Uniprot.org