• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from 293 cells treated with TNF-a 20ng/ml 30', using IkappaB-beta (Phospho-Thr19) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 293 cells using Phospho-I Kappa B-Beta (T19) Polyclonal Antibody

Anti-Phospho-NFKBIB-Thr19 antibody (4-53 aa) (STJ90460)

SKU:
STJ90460

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-NF-kappa-B inhibitor beta-Thr19 (4-53 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFKBIB
Gene ID: 4793
Uniprot ID: IKBB_HUMAN
Immunogen Region: 4-53 aa
Specificity: Phospho-I Kappa B-Beta (T19) Polyclonal Antibody detects endogenous levels of I Kappa B-Beta protein only when phosphorylated at T19.
Immunogen: The antiserum was produced against synthesized peptide derived from the human IkappaB-beta around the phosphorylation site of Thr19 at the amino acid range 4-53
Post Translational Modifications Phosphorylated by RPS6KA1.followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
Function Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
Protein Name Nf-Kappa-B Inhibitor Beta
Nf-Kappa-Bib
I-Kappa-B-Beta
Ikb-B
Ikb-Beta
Ikappabbeta
Thyroid Receptor-Interacting Protein 9
Tr-Interacting Protein 9
Trip-9
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1810476
Reactome: R-HSA-445989
Reactome: R-HSA-933542
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Nf-Kappa-B Inhibitor Beta antibody
Anti-Nf-Kappa-Bib antibody
Anti-I-Kappa-B-Beta antibody
Anti-Ikb-B antibody
Anti-Ikb-Beta antibody
Anti-Ikappabbeta antibody
Anti-Thyroid Receptor-Interacting Protein 9 antibody
Anti-Tr-Interacting Protein 9 antibody
Anti-Trip-9 antibody
Anti-NFKBIB antibody
Anti-IKBB antibody
Anti-TRIP9 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance