Anti-Phospho-NFATC1-Ser294 antibody (261-310 aa) (STJ91118)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human NFAT2 around the phosphorylation site of Ser294 at the amino acid range 261-310
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Nuclear factor of activated T-cells, cytoplasmic 1-Ser294 (261-310 aa) for use in IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NFATC1 |
| Gene ID | 4772 |
| Uniprot ID | NFAC1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human NFAT2 around the phosphorylation site of Ser294 at the amino acid range 261-310 |
| Immunogen Region | 261-310 aa |
| Specificity | Phospho-NFATc1 (S294) Polyclonal Antibody detects endogenous levels of NFATc1 protein only when phosphorylated at S294. |
Additional Info
| Post Translational Modifications | Phosphorylated by NFATC-kinase and GSK3B.phosphorylation induces NFATC1 nuclear exit and dephosphorylation by calcineurin promotes nuclear import. Phosphorylation by PKA and DYRK2 negatively modulates nuclear accumulation, and promotes subsequent phosphorylation by GSK3B or casein kinase 1. |
| Function | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function. |
| Protein Name | Nuclear Factor Of Activated T-Cells - Cytoplasmic 1Nf-Atc1Nfatc1Nfat Transcription Complex Cytosolic ComponentNf-AtcNfatc |
| Database Links | Reactome: R-HSA-2025928Reactome: R-HSA-2871809Reactome: R-HSA-4086398Reactome: R-HSA-5607763 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic For The Phosphorylated Form And Nuclear After Activation That Is Controlled By Calcineurin-Mediated DephosphorylationRapid Nuclear Exit Of Nfatc Is Thought To Be One Mechanism By Which Cells Distinguish Between Sustained And Transient Calcium SignalsTranslocation To The Nucleus Is Increased In The Presence Of Calcium In Pre-OsteoblastsThe Subcellular Localization Of Nfatc Plays A Key Role In The Regulation Of Gene TranscriptionNuclear Translocation Of Nfatc1 Is Enhanced In The Presence Of Tnfsf11Nuclear Translocation Is Decreased In The Presence Of Fbn1 Which Can Bind And Sequester Tnfsf11 |
| Alternative Antibody Names | Anti-Nuclear Factor Of Activated T-Cells - Cytoplasmic 1 antibodyAnti-Nf-Atc1 antibodyAnti-Nfatc1 antibodyAnti-Nfat Transcription Complex Cytosolic Component antibodyAnti-Nf-Atc antibodyAnti-Nfatc antibodyAnti-NFATC1 antibodyAnti-NFAT2 antibodyAnti-NFATC antibody |
Information sourced from Uniprot.org