• Immunohistochemistry analysis of Phospho-Nucleolin-T76 in paraffin-embedded human lung cancer using Phospho-Nucleolin-T76 Rabbit monoclonal antibody (STJ11105810) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-Nucleolin-T76 in paraffin-embedded human esophagus using Phospho-Nucleolin-T76 Rabbit monoclonal antibody (STJ11105810) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-NCL-T76 antibody [S5810RM] (STJ11105810)

SKU:
STJ11105810

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-Nucleolin-T76 is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S5810RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCL
Gene ID: 4691
Uniprot ID: NUCL_HUMAN
Immunogen: A phospho synthetic peptide corresponding to residues surrounding T76 of Human Nucleolin.
Post Translational Modifications Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. Symmetrically methylated by PRMT5.
Function Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Protein Name Nucleolin
Protein C23
Database Links Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Nucleolin antibody
Anti-Protein C23 antibody
Anti-NCL antibody

Information sourced from Uniprot.org

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