Anti-Phospho-NCF4-Thr154 antibody (120-169 aa) (STJ91131)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human p40 phox around the phosphorylation site of Thr154 at the amino acid range 120-169
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Neutrophil cytosol factor 4-Thr154 (120-169 aa) for use in IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NCF4 |
| Gene ID | 4689 |
| Uniprot ID | NCF4_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human p40 phox around the phosphorylation site of Thr154 at the amino acid range 120-169 |
| Immunogen Region | 120-169 aa |
| Specificity | Phospho-p40-phox (T154) Polyclonal Antibody detects endogenous levels of p40-phox protein only when phosphorylated at T154. |
Additional Info
| Function | Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (Probable). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. |
| Protein Name | Neutrophil Cytosol Factor 4Ncf-4Neutrophil Nadph Oxidase Factor 4Sh3 And Px Domain-Containing Protein 4P40-PhoxP40phox |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1236973Reactome: R-HSA-3299685Reactome: R-HSA-4420097Reactome: R-HSA-5668599Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423 |
| Cellular Localisation | CytoplasmCytosolEndosome MembranePeripheral Membrane ProteinCytoplasmic SideMembraneTranslocates To The Membrane Upon Activation By Phorbol Myristate Acetate (Pma) |
| Alternative Antibody Names | Anti-Neutrophil Cytosol Factor 4 antibodyAnti-Ncf-4 antibodyAnti-Neutrophil Nadph Oxidase Factor 4 antibodyAnti-Sh3 And Px Domain-Containing Protein 4 antibodyAnti-P40-Phox antibodyAnti-P40phox antibodyAnti-NCF4 antibodyAnti-SH3PXD4 antibody |
Information sourced from Uniprot.org