• Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-NACA-Ser43 antibody (9-58 aa) (STJ91263)

SKU:
STJ91263

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Nascent polypeptide-associated complex subunit alpha-Ser43 (9-58 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NACA
Gene ID: 4666
Uniprot ID: NACA_HUMAN
Immunogen Region: 9-58 aa
Specificity: Phospho-NACA (S43) Polyclonal Antibody detects endogenous levels of NACA protein only when phosphorylated at S43.
Immunogen: The antiserum was produced against synthesized peptide derived from the human NACA around the phosphorylation site of Ser43 at the amino acid range 9-58
Post Translational Modifications Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation. Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization.
Function Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
Protein Name Nascent Polypeptide-Associated Complex Subunit Alpha
Nac-Alpha
Alpha-Nac
Allergen Hom S 2
Cellular Localisation Cytoplasm
Nucleus
The Heterodimer Is Located Mainly In The Cytosol
And The Homodimer In The Nucleus
Alternative Antibody Names Anti-Nascent Polypeptide-Associated Complex Subunit Alpha antibody
Anti-Nac-Alpha antibody
Anti-Alpha-Nac antibody
Anti-Allergen Hom S 2 antibody
Anti-NACA antibody
Anti-HSD48 antibody

Information sourced from Uniprot.org

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