Anti-Phospho-MUSK-Tyr755 antibody (STJ90670)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phospho-peptide around the phosphorylation site of human MuSK (phospho Tyr755)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Muscle, skeletal receptor tyrosine-protein kinase-Tyr755 for use in WB, ELISA and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MUSK |
| Gene ID | 4593 |
| Uniprot ID | MUSK_HUMAN |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human MuSK (phospho Tyr755) |
| Specificity | Phospho-MuSK (Y755) Polyclonal Antibody detects endogenous levels of MuSK protein only when phosphorylated at Y755. |
Additional Info
| Function | Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation. |
| Protein Name | Muscle - Skeletal Receptor Tyrosine-Protein KinaseMuscle-Specific Tyrosine-Protein Kinase ReceptorMuskMuscle-Specific Kinase Receptor |
| Database Links | Reactome: R-HSA-3000178 |
| Cellular Localisation | Postsynaptic Cell MembraneSingle-Pass Type I Membrane ProteinColocalizes With Acetylcholine Receptors (Achr) To The Postsynaptic Cell Membrane Of The Neuromuscular Junction |
| Alternative Antibody Names | Anti-Muscle - Skeletal Receptor Tyrosine-Protein Kinase antibodyAnti-Muscle-Specific Tyrosine-Protein Kinase Receptor antibodyAnti-Musk antibodyAnti-Muscle-Specific Kinase Receptor antibodyAnti-MUSK antibody |
Information sourced from Uniprot.org