| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Moesin and Radixin and Ezrin-Thr558 (524-573 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RDXEZR
|
| Gene ID: |
596274304478RADI_HUMANEZRI_HUMANMOES_HUMAN
|
| Immunogen Region: |
524-573 aa |
| Specificity: |
Phospho-Moesin/Ezrin/Radixin (T558) Polyclonal Antibody detects endogenous levels of Moesin/Ezrin/Radixin protein only when phosphorylated at T558. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Moesin/Ezrin/Radixin around the phosphorylation site of Thr558 at the amino acid range 524-573 |
| Post Translational Modifications | Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding. Phosphorylation on Thr-558 by STK10 negatively regulates lymphocyte migration and polarization. S-nitrosylation of Cys-117 is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) implicating the iNOS-S100A8/9 transnitrosylase complex. |
| Function | Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton. Once phosphorylated on its C-terminal threonine, moesin is activated leading to interaction with F-actin and cytoskeletal rearrangement. These rearrangements regulate many cellular processes, including cell shape determination, membrane transport, and signal transduction. The role of moesin is particularly important in immunity acting on both T and B-cells homeostasis and self-tolerance, regulating lymphocyte egress from lymphoid organs. Modulates phagolysosomal biogenesis in macrophages. Participates also in immunologic synapse formation. |
| Protein Name | MoesinMembrane-Organizing Extension Spike Protein |
| Database Links | Reactome: R-HSA-437239Reactome: R-HSA-8950505 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmCytoskeletonApical Cell MembraneCell ProjectionMicrovillus MembraneMicrovillusPhosphorylated Form Is Enriched In Microvilli-Like Structures At Apical MembraneIncreased Cell Membrane Localization Of Both Phosphorylated And Non-Phosphorylated Forms Seen After Thrombin TreatmentLocalizes At The Uropods Of T Lymphoblasts |
| Alternative Antibody Names | Anti-Moesin antibodyAnti-Membrane-Organizing Extension Spike Protein antibodyAnti-MSN antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
