• Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-MOB1A-Thr12 antibody (STJ194726)

SKU:
STJ194726

Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MOB kinase activator 1A-Thr12 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MOB1A
Gene ID: 55233
Uniprot ID: MOB1A_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse Rat MOB1 (phospho-Thr12)
Immunogen: Synthesized phosho peptide around human MOB1 (Thr12)
Post Translational Modifications Phosphorylated by STK3/MST2 and STK4/MST1 and this phosphorylation enhances its binding to LATS1.
Function Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38.
Protein Name Mob Kinase Activator 1a
Mob1 Alpha
Mob1a
Mob1 Homolog 1b
Mps One Binder Kinase Activator-Like 1b
Database Links Reactome: R-HSA-2028269
Alternative Antibody Names Anti-Mob Kinase Activator 1a antibody
Anti-Mob1 Alpha antibody
Anti-Mob1a antibody
Anti-Mob1 Homolog 1b antibody
Anti-Mps One Binder Kinase Activator-Like 1b antibody
Anti-MOB1A antibody
Anti-C2orf6 antibody
Anti-MOB4B antibody
Anti-MOBK1B antibody
Anti-MOBKL1B antibody

Information sourced from Uniprot.org

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