• Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ug/ml 24h, using MNK1 (Phospho-Thr255) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MKNK1-Thr255 antibody (221-270 aa) (STJ90587)

SKU:
STJ90587

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MAP kinase-interacting serine/threonine-protein kinase 1-Thr255 (221-270 aa) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MKNK1
Gene ID: 8569
Uniprot ID: MKNK1_HUMAN
Immunogen Region: 221-270 aa
Specificity: Phospho-Mnk1 (T255) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T255.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MNK1 around the phosphorylation site of Thr255 at the amino acid range 221-270
Post Translational Modifications Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.
Function May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Protein Name Map Kinase-Interacting Serine/Threonine-Protein Kinase 1
Map Kinase Signal-Integrating Kinase 1
Mapk Signal-Integrating Kinase 1
Mnk1
Database Links Reactome: R-HSA-1295596
Cellular Localisation Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Nucleus
Alternative Antibody Names Anti-Map Kinase-Interacting Serine/Threonine-Protein Kinase 1 antibody
Anti-Map Kinase Signal-Integrating Kinase 1 antibody
Anti-Mapk Signal-Integrating Kinase 1 antibody
Anti-Mnk1 antibody
Anti-MKNK1 antibody
Anti-MNK1 antibody

Information sourced from Uniprot.org

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