• Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MNK1 (Phospho-Thr250) Antibody
  • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Phospho-MKNK1-Thr250 antibody (216-265 aa) (STJ91211)

SKU:
STJ91211

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MAP kinase-interacting serine/threonine-protein kinase 1-Thr250 (216-265 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MKNK1
Gene ID: 8569
Uniprot ID: MKNK1_HUMAN
Immunogen Region: 216-265 aa
Specificity: Phospho-Mnk1 (T250) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T250.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MNK1 around the phosphorylation site of Thr250 at the amino acid range 216-265
Post Translational Modifications Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.
Function May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Protein Name Map Kinase-Interacting Serine/Threonine-Protein Kinase 1
Map Kinase Signal-Integrating Kinase 1
Mapk Signal-Integrating Kinase 1
Mnk1
Database Links Reactome: R-HSA-1295596
Cellular Localisation Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Nucleus
Alternative Antibody Names Anti-Map Kinase-Interacting Serine/Threonine-Protein Kinase 1 antibody
Anti-Map Kinase Signal-Integrating Kinase 1 antibody
Anti-Mapk Signal-Integrating Kinase 1 antibody
Anti-Mnk1 antibody
Anti-MKNK1 antibody
Anti-MNK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance