• Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MCM3-Ser112 antibody (STJ196354)

SKU:
STJ196354

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-DNA replication licensing factor MCM3-Ser112 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCM3
Gene ID: 4172
Uniprot ID: MCM3_HUMAN
Specificity: This antibody detects endogenous levels of Human MCM3 (phospho-Ser112)
Immunogen: Synthesized phosho peptide around human MCM3 (Ser112)
Function Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division (Probable).
Protein Name Dna Replication Licensing Factor Mcm3
Dna Polymerase Alpha Holoenzyme-Associated Protein P1
P1-Mcm3
Rlf Subunit Beta
P102
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-176974
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-68962
Reactome: R-HSA-69052
Cellular Localisation Nucleus
Chromosome
Associated With Chromatin Before The Formation Of Nuclei And Detaches From It As Dna Replication Progresses
Alternative Antibody Names Anti-Dna Replication Licensing Factor Mcm3 antibody
Anti-Dna Polymerase Alpha Holoenzyme-Associated Protein P1 antibody
Anti-P1-Mcm3 antibody
Anti-Rlf Subunit Beta antibody
Anti-P102 antibody
Anti-MCM3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance