• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-MCL1-T163 antibody (STJ11101076) at 1:1000 dilution. NIH/3T3 cells were treated by PMA (80 nM) for overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-MCL1-T163 Rabbit polyclonal antibody (STJ11101076) at 1:1000 dilution. NIH/3T3 cells were treated by PMA (80 nM) for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-MCL1-T163 antibody (STJ11101076)

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STJ11101076

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MCL1-T163 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCL1
Gene ID: 4170
Uniprot ID: MCL1_HUMAN
Immunogen: A synthetic phosphorylated peptide around T163 of human MCL1 (NP_068779.1).
Immunogen Sequence: PSTPP
Post Translational Modifications Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment. Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region. Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159. Deubiquitinated by USP20.leading to increased stability.
Function Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Protein Name Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1
Bcl-2-Like Protein 3
Bcl2-L-3
Bcl-2-Related Protein Eat/Mcl1
Mcl1/Eat
Database Links Reactome: R-HSA-6785807
Cellular Localisation Membrane
Single-Pass Membrane Protein
Cytoplasm
Mitochondrion
Nucleus
Nucleoplasm
Cytoplasmic
Associated With Mitochondria
Alternative Antibody Names Anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 antibody
Anti-Bcl-2-Like Protein 3 antibody
Anti-Bcl2-L-3 antibody
Anti-Bcl-2-Related Protein Eat/Mcl1 antibody
Anti-Mcl1/Eat antibody
Anti-MCL1 antibody
Anti-BCL2L3 antibody

Information sourced from Uniprot.org

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