| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Phospho-MAP kinase-activated protein kinase 2-Thr222 (188-237 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAPKAPK2 |
| Gene ID: | 9261 |
| Uniprot ID: | MAPK2_HUMAN |
| Immunogen Region: | 188-237 aa |
| Specificity: | Phospho-MAPKAPK-2 (T222) Polyclonal Antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at T222. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human MAPKAPK-2 around the phosphorylation site of Thr222 at the amino acid range 188-237 |
| Post Translational Modifications | Sumoylation inhibits the protein kinase activity. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334. |
| Function | Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1.leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. |
| Protein Name | Map Kinase-Activated Protein Kinase 2Mapk-Activated Protein Kinase 2Mapkap Kinase 2Mapkap-K2Mapkapk-2Mk-2Mk2 |
| Database Links | Reactome: R-HSA-171007Reactome: R-HSA-199920Reactome: R-HSA-2142691Reactome: R-HSA-2559580Reactome: R-HSA-3371453Reactome: R-HSA-4420097Reactome: R-HSA-450302Reactome: R-HSA-450385Reactome: R-HSA-450513Reactome: R-HSA-5357905 |
| Cellular Localisation | CytoplasmNucleusPhosphorylation And Subsequent Activation Releases The Autoinhibitory HelixResulting In The Export From The Nucleus Into The Cytoplasm |
| Alternative Antibody Names | Anti-Map Kinase-Activated Protein Kinase 2 antibodyAnti-Mapk-Activated Protein Kinase 2 antibodyAnti-Mapkap Kinase 2 antibodyAnti-Mapkap-K2 antibodyAnti-Mapkapk-2 antibodyAnti-Mk-2 antibodyAnti-Mk2 antibodyAnti-MAPKAPK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
