Anti-Phospho-MAP3K8-Ser400 antibody (366-415 aa) (STJ91209)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human MAP3K8 around the phosphorylation site of Ser400 at the amino acid range 366-415
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Mitogen-activated protein kinase kinase kinase 8-Ser400 (366-415 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAP3K8 |
| Gene ID | 1326 |
| Uniprot ID | M3K8_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human MAP3K8 around the phosphorylation site of Ser400 at the amino acid range 366-415 |
| Immunogen Region | 366-415 aa |
| Specificity | Phospho-Cot (S400) Polyclonal Antibody detects endogenous levels of Cot protein only when phosphorylated at S400. |
Additional Info
| Post Translational Modifications | Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. Phosphorylated on Thr-290.the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation. Phosphorylated on Ser-400 by IKBKB.the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages. |
| Function | Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 8Cancer Osaka Thyroid OncogeneProto-Oncogene C-CotSerine/Threonine-Protein Kinase CotTumor Progression Locus 2Tpl-2 |
| Database Links | Reactome: R-HSA-389357Reactome: R-HSA-5684264 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 antibodyAnti-Cancer Osaka Thyroid Oncogene antibodyAnti-Proto-Oncogene C-Cot antibodyAnti-Serine/Threonine-Protein Kinase Cot antibodyAnti-Tumor Progression Locus 2 antibodyAnti-Tpl-2 antibodyAnti-MAP3K8 antibodyAnti-COT antibodyAnti-ESTF antibody |
Information sourced from Uniprot.org