Anti-Phospho-MAP3K11-Ser674 antibody (STJ91362)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phospho-peptide around the phosphorylation site of human MLK3 (phospho Ser674)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Mitogen-activated protein kinase kinase kinase 11-Ser674 for use in WB and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAP3K11 |
| Gene ID | 4296 |
| Uniprot ID | M3K11_HUMAN |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human MLK3 (phospho Ser674) |
| Specificity | Phospho-MLK3 (S674) Polyclonal Antibody detects endogenous levels of MLK3 protein only when phosphorylated at S674. |
Additional Info
| Post Translational Modifications | Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42. |
| Function | Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 11Mixed Lineage Kinase 3Src-Homology 3 Domain-Containing Proline-Rich Kinase |
| Database Links | Reactome: R-HSA-5673000Reactome: R-HSA-6802946Reactome: R-HSA-6802955Reactome: R-HSA-9013148Reactome: R-HSA-9013408Reactome: R-HSA-9013424Reactome: R-HSA-9649948 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeLocation Is Cell Cycle Dependent |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 11 antibodyAnti-Mixed Lineage Kinase 3 antibodyAnti-Src-Homology 3 Domain-Containing Proline-Rich Kinase antibodyAnti-MAP3K11 antibodyAnti-MLK3 antibodyAnti-PTK1 antibodyAnti-SPRK antibody |
Information sourced from Uniprot.org