| Host: |
Rabbit |
| Applications: |
IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Dual specificity mitogen-activated protein kinase kinase 4-Ser257 is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAP2K4 |
| Gene ID: |
6416 |
| Uniprot ID: |
MP2K4_HUMAN |
| Specificity: |
Phospho-MEK-4 (S257) Polyclonal Antibody detects endogenous levels of MEK-4 protein only when phosphorylated at S257. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human SEK1/MKK4/JNKK1 around the phosphorylation site of Ser257. the amino acid range:223-272 |
| Post Translational Modifications | Activated by phosphorylation on Ser-257 and Thr-261 by MAP kinase kinase kinases (MAP3Ks). |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to pro-inflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 4Map Kinase Kinase 4Mapkk 4Jnk-Activating Kinase 1Mapk/Erk Kinase 4Mek 4Sapk/Erk Kinase 1Sek1Stress-Activated Protein Kinase Kinase 1Sapk Kinase 1Sapkk-1Sapkk1C-Jun N-Terminal Kinase Kinase 1Jnkk |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-2871796Reactome: R-HSA-450321Reactome: R-HSA-5210891Reactome: R-HSA-5684264 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 4 antibodyAnti-Map Kinase Kinase 4 antibodyAnti-Mapkk 4 antibodyAnti-Jnk-Activating Kinase 1 antibodyAnti-Mapk/Erk Kinase 4 antibodyAnti-Mek 4 antibodyAnti-Sapk/Erk Kinase 1 antibodyAnti-Sek1 antibodyAnti-Stress-Activated Protein Kinase Kinase 1 antibodyAnti-Sapk Kinase 1 antibodyAnti-Sapkk-1 antibodyAnti-Sapkk1 antibodyAnti-C-Jun N-Terminal Kinase Kinase 1 antibodyAnti-Jnkk antibodyAnti-MAP2K4 antibodyAnti-JNKK1 antibodyAnti-MEK4 antibodyAnti-MKK4 antibodyAnti-PRKMK4 antibodyAnti-SEK1 antibodyAnti-SERK1 antibodyAnti-SKK1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
