Western blot analysis of lysates from NIH/3T3 cells, using Phospho-MAP2K3-S189 + MAP2K6-S207 Rabbit pAb (STJ11101146) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MAP2K3/MAP2K6-S189/S207 antibody (STJ11101146)

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STJ11101146
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Short Description :Rabbit polyclonal Phospho-MAP2K3/MAP2K6-S189/S207 antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/ELISA
Host:Rabbit
Reactivity:Human/Mouse
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:MAP2K3
MAP2K6
Gene ID:5606
5608
Uniprot ID:MP2K3_HUMAN
MP2K6_HUMAN
Immunogen Sequence:VDSVA
Specificity:A synthetic phosphorylated peptide around S207 of human MAP2K3/MAP2K6MAP2K3 (NP_002747.2).
Tissue Specificity P46734 - Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
P52564 - Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 3 antibody
Anti-Map Kinase Kinase 3 antibody
Anti-Mapkk 3 antibody
Anti-Mapk/Erk Kinase 3 antibody
Anti-Mek 3 antibody
Anti-Stress-Activated Protein Kinase Kinase 2 antibody
Anti-Sapk Kinase 2 antibody
Anti-Sapkk-2 antibody
Anti-Sapkk2 antibody
Anti-MAP2K3 antibody
Anti-MEK3 antibody
Anti-MKK3 antibody
Anti-PRKMK3 antibody
Anti-SKK2 antibody
Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 6 antibody
Anti-Map Kinase Kinase 6 antibody
Anti-Mapkk 6 antibody
Anti-Mapk/Erk Kinase 6 antibody
Anti-Mek 6 antibody
Anti-Stress-Activated Protein Kinase Kinase 3 antibody
Anti-Sapk Kinase 3 antibody
Anti-Sapkk-3 antibody
Anti-Sapkk3 antibody
Anti-MAP2K6 antibody
Anti-MEK6 antibody
Anti-MKK6 antibody
Anti-PRKMK6 antibody
Anti-SKK3 antibody

Information sourced from Uniprot.org

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