• Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using MAK (Phospho-Tyr159) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAK-Tyr159 antibody (126-175 aa) (STJ90583)

SKU:
STJ90583

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase MAK-Tyr159 (126-175 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAK
Gene ID: 4117
Uniprot ID: MAK_HUMAN
Immunogen Region: 126-175 aa
Specificity: Phospho-MAK (Y159) Polyclonal Antibody detects endogenous levels of MAK protein only when phosphorylated at Y159.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MAK around the phosphorylation site of Tyr159 at the amino acid range 126-175
Post Translational Modifications Autophosphorylated. Phosphorylated on serine and threonine residues.
Function Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors. Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
Protein Name Serine/Threonine-Protein Kinase Mak
Male Germ Cell-Associated Kinase
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Midbody
Cell Projection
Cilium
Photoreceptor Outer Segment
Photoreceptor Inner Segment
Localized In Both The Connecting Cilia And The Outer Segment Axonemes
Localized Uniformly In Nuclei During Interphase
To The Mitotic Spindle And Centrosomes During Metaphase And Anaphase
And Also To Midbody At Anaphase Until Telophase
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Mak antibody
Anti-Male Germ Cell-Associated Kinase antibody
Anti-MAK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance