• Western blot analysis of extracts of various cell lines, using Phospho-HSL-S563 antibody (STJ11100992) at 1:1000 dilution. 3T3L1 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. H9C2 cells were treated with Isoproterenol (10 Mu M for 20 min) at 37 °C. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using Phospho-HSL-S563 Rabbit polyclonal antibody (STJ11100992) at 1:1000 dilution. 3T3L1 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. H9C2 cells were treated with Isoproterenol (10 Mu M for 20 min) at 37 °C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-LIPE-S563 antibody (STJ11100992)

SKU:
STJ11100992

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-LIPE-S563 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIPE
Gene ID: 3991
Uniprot ID: LIPS_HUMAN
Immunogen: A synthetic phosphorylated peptide around S563 of rat HSL (XP_006228456.1).
Immunogen Sequence: RRSVS
Tissue Specificity Testis.
Post Translational Modifications Phosphorylation by AMPK reduces its translocation towards the lipid droplets.
Function Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Protein Name Hormone-Sensitive Lipase
Hsl
Monoacylglycerol Lipase Lipe
Retinyl Ester Hydrolase
Reh
Database Links Reactome: R-HSA-163560
Cellular Localisation Cell Membrane
Membrane
Caveola
Cytoplasm
Cytosol
Lipid Droplet
Found In The High-Density Caveolae
Translocates To The Cytoplasm From The Caveolae Upon Insulin Stimulation
Phosphorylation By Ampk Reduces Its Translocation Towards The Lipid Droplets
Alternative Antibody Names Anti-Hormone-Sensitive Lipase antibody
Anti-Hsl antibody
Anti-Monoacylglycerol Lipase Lipe antibody
Anti-Retinyl Ester Hydrolase antibody
Anti-Reh antibody
Anti-LIPE antibody

Information sourced from Uniprot.org

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