Anti-Phospho-LIMK-1/2-Thr508/505 antibody (STJ90751)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phospho-peptide around the phosphorylation site of human LIMK-1/2 (phospho Thr508/505)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-LIM domain kinase 1 and LIM domain kinase 2-Thr508/505 for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:100-500IF ICC 1:100-500ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LIMK2LIMK1 |
| Gene ID | 39853984 |
| Uniprot ID | LIMK2_HUMANLIMK1_HUMAN |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human LIMK-1/2 (phospho Thr508/505) |
| Specificity | Phospho-LIMK-1/2 (T508/505) Polyclonal Antibody detects endogenous levels of LIMK-1/2 protein only when phosphorylated at T508/505. |
Additional Info
| Post Translational Modifications | Autophosphorylated. Phosphorylated on Thr-508 by ROCK1 and PAK1, resulting in activation. Phosphorylated by PAK4 which increases the ability of LIMK1 to phosphorylate cofilin. Phosphorylated at Ser-323 by MAPKAPK2 during activation of VEGFA-induced signaling, which results in activation of LIMK1 and promotion of actin reorganization, cell migration, and tubule formation of endothelial cells. Dephosphorylated and inactivated by SSH1. Phosphorylated by CDC42BP. Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM. |
| Function | Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3: Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1). |
| Protein Name | Lim Domain Kinase 1Limk-1 |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-3928662Reactome: R-HSA-399954Reactome: R-HSA-416572Reactome: R-HSA-5627117Reactome: R-HSA-5627123 |
| Cellular Localisation | CytoplasmNucleusCytoskeletonCell ProjectionLamellipodiumPredominantly Found In The CytoplasmLocalizes In The Lamellipodium In A Cdc42bpaCdc42bpb And Fam89b/Lrap25-Dependent Manner |
| Alternative Antibody Names | Anti-Lim Domain Kinase 1 antibodyAnti-Limk-1 antibodyAnti-LIMK1 antibodyAnti-LIMK antibody |
Information sourced from Uniprot.org