Anti-Phospho-LATS1/2-Thr1079/1041 antibody (1041-1090 aa) (STJ91207)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human LATS1/2 around the phosphorylation site of Thr1079/1041 at the amino acid range 1041-1090
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Serine/threonine-protein kinase LATS1 and Serine/threonine-protein kinase LATS2-Thr1079/1041 (1041-1090 aa) for use in IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LATS1LATS2 |
| Gene ID | 911326524 |
| Uniprot ID | LATS1_HUMANLATS2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human LATS1/2 around the phosphorylation site of Thr1079/1041 at the amino acid range 1041-1090 |
| Immunogen Region | 1041-1090 aa |
| Specificity | Phospho-LATS1/2 (T1079/1041) Polyclonal Antibody detects endogenous levels of LATS1/2 protein only when phosphorylated at T1079/1041. |
Additional Info
| Post Translational Modifications | Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy. |
| Function | Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cell differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1. |
| Protein Name | Serine/Threonine-Protein Kinase Lats1Large Tumor Suppressor Homolog 1Warts Protein KinaseH-Warts |
| Database Links | Reactome: R-HSA-2028269 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindleMidbodySpindle Pole BodyLocalizes To The Centrosomes Throughout Interphase But Migrates To The Mitotic ApparatusIncluding Spindle Pole BodiesMitotic SpindleAnd MidbodyDuring Mitosis |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Lats1 antibodyAnti-Large Tumor Suppressor Homolog 1 antibodyAnti-Warts Protein Kinase antibodyAnti-H-Warts antibodyAnti-LATS1 antibodyAnti-WARTS antibody |
Information sourced from Uniprot.org