• Western blot analysis of lysates from NIH/3T3 cells, using LAT (Phospho-Tyr191) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using LAT (Phospho-Tyr191) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-LAT-Tyr191 antibody (191-240 aa) (STJ90819)

SKU:
STJ90819

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Linker for activation of T-cells family member 1-Tyr191 (191-240 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LAT
Gene ID: 27040
Uniprot ID: LAT_HUMAN
Immunogen Region: 191-240 aa
Specificity: Phospho-LAT (Y191) Polyclonal Antibody detects endogenous levels of LAT protein only when phosphorylated at Y191.
Immunogen: The antiserum was produced against synthesized peptide derived from the human LAT around the phosphorylation site of Tyr191 at the amino acid range 191-240
Post Translational Modifications Phosphorylated on tyrosines by ZAP70 upon TCR activation, or by SYK upon other immunoreceptor activation.which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Phosphorylated by ITK leading to the recruitment of VAV1 to LAT-containing complexes. Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation. 'Lys-63'-linked ubiquitinated by TRAF6.
Function Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
Protein Name Linker For Activation Of T-Cells Family Member 1
36 Kda Phosphotyrosine Adapter Protein
Pp36
P36-38
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-202433
Reactome: R-HSA-2424491 O43561-2
Reactome: R-HSA-2454202 O43561-2
Reactome: R-HSA-2871796 O43561-2
Reactome: R-HSA-2871809 O43561-2
Reactome: R-HSA-5673001 O43561-2
Cellular Localisation Cell Membrane
Single-Pass Type Iii Membrane Protein
Present In Lipid Rafts
Alternative Antibody Names Anti-Linker For Activation Of T-Cells Family Member 1 antibody
Anti-36 Kda Phosphotyrosine Adapter Protein antibody
Anti-Pp36 antibody
Anti-P36-38 antibody
Anti-LAT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance