• Western blot analysis of lysates from Hep G2, using Phospho-Cytokeratin 8 (KRT8) -S74 Rabbit polyclonal antibody (STJ11105809) at 1:1000 dilution. Hep G2 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-KRT8-S74 antibody (STJ11105809)

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STJ11105809

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Keratin-Type Ii Cytoskeletal 8-S74 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT8
Gene ID: 3856
Uniprot ID: K2C8_HUMAN
Immunogen: A synthetic phosphorylated peptide around S74 of human Cytokeratin 8 (NP_002264.1).
Immunogen Sequence: LLSPL
Tissue Specificity Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Post Translational Modifications Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Protein Name Keratin - Type Ii Cytoskeletal 8
Cytokeratin-8
Ck-8
Keratin-8
K8
Type-Ii Keratin Kb8
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleus Matrix
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 8 antibody
Anti-Cytokeratin-8 antibody
Anti-Ck-8 antibody
Anti-Keratin-8 antibody
Anti-K8 antibody
Anti-Type-Ii Keratin Kb8 antibody
Anti-KRT8 antibody
Anti-CYK8 antibody

Information sourced from Uniprot.org

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