| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase 3 and Serine/threonine-protein kinase 4-Thr183 (149-198 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
STK4STK3 |
| Gene ID: |
67896788 |
| Uniprot ID: |
STK4_HUMANSTK3_HUMAN |
| Immunogen Region: |
149-198 aa |
| Specificity: |
Phospho-Krs-1/2 (T183) Polyclonal Antibody detects endogenous levels of Krs-1/2 protein only when phosphorylated at T183. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Mst1/2 around the phosphorylation site of Thr183 at the amino acid range 149-198 |
| Post Translational Modifications | Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1. Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N). |
| Function | Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38. |
| Protein Name | Serine/Threonine-Protein Kinase 3Mammalian Ste20-Like Protein Kinase 2Mst-2Ste20-Like Kinase Mst2Serine/Threonine-Protein Kinase Krs-1 Cleaved Into - Serine/Threonine-Protein Kinase 3 36kda SubunitMst2/N - Serine/Threonine-Protein Kinase 3 20kda SubunitMst2/C |
| Database Links | Reactome: R-HSA-2028269 |
| Cellular Localisation | CytoplasmNucleusThe Caspase-Cleaved Form Cycles Between Nucleus And CytoplasmPhosphorylation At Thr-117 Leads To Inhibition Of Nuclear Translocation |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase 3 antibodyAnti-Mammalian Ste20-Like Protein Kinase 2 antibodyAnti-Mst-2 antibodyAnti-Ste20-Like Kinase Mst2 antibodyAnti-Serine/Threonine-Protein Kinase Krs-1 Cleaved Into - Serine/Threonine-Protein Kinase 3 36kda Subunit antibodyAnti-Mst2/N - Serine/Threonine-Protein Kinase 3 20kda Subunit antibodyAnti-Mst2/C antibodyAnti-STK3 antibodyAnti-KRS1 antibodyAnti-MST2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
