• Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit monoclonal antibody (STJ11105826, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Confocal imaging of paraffin-embedded Mouse brain tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit monoclonal antibody (STJ11105826, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Immunohistochemistry analysis of Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 in paraffin-embedded Rat colon tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit monoclonal antibody (STJ11105826) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 in paraffin-embedded Rat brain tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit monoclonal antibody (STJ11105826) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 in paraffin-embedded Mouse kidney tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit monoclonal antibody (STJ11105826) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 in paraffin-embedded Mouse brain tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit monoclonal antibody (STJ11105826) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates, using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit monoclonal antibody (STJ11105826) at1:2000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates, using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit monoclonal antibody (STJ11105826) at1:2000 dilution. 293T and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-JNK1 + JNK2 + JNK3-T183/Y185-T183/Y185-T221/Y223 antibody [S5826RM] (STJ11105826)

SKU:
STJ11105826

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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5826RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK8
MAPK9
5599
5601
5602
MK08_HUMAN
MK09_HUMAN
MK10_HUMAN
Specificity: A synthetic phosphorylated peptide around T183 of human MAPK8 (NP_620637.1).
Immunogen Sequence: MMTPYVV
Tissue Specificity P45983 - NA
P45984 - NA
P53779 - Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
P45983 - NA
P45984 - NA
P53779 - Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 8 antibody
Anti-Map Kinase 8 antibody
Anti-Mapk 8 antibody
Anti-Jnk-46 antibody
Anti-Stress-Activated Protein Kinase 1c antibody
Anti-Sapk1c antibody
Anti-Stress-Activated Protein Kinase Jnk1 antibody
Anti-C-Jun N-Terminal Kinase 1 antibody
Anti-MAPK8 antibody
Anti-JNK1 antibody
Anti-PRKM8 antibody
Anti-SAPK1 antibody
Anti-SAPK1C antibody
Anti-Mitogen-Activated Protein Kinase 9 antibody
Anti-Map Kinase 9 antibody
Anti-Mapk 9 antibody
Anti-Jnk-55 antibody
Anti-Stress-Activated Protein Kinase 1a antibody
Anti-Sapk1a antibody
Anti-Stress-Activated Protein Kinase Jnk2 antibody
Anti-C-Jun N-Terminal Kinase 2 antibody
Anti-MAPK9 antibody
Anti-JNK2 antibody
Anti-PRKM9 antibody
Anti-SAPK1A antibody
Anti-Mitogen-Activated Protein Kinase 10 antibody
Anti-Map Kinase 10 antibody
Anti-Mapk 10 antibody
Anti-Map Kinase P49 3f12 antibody
Anti-Stress-Activated Protein Kinase 1b antibody
Anti-Sapk1b antibody
Anti-Stress-Activated Protein Kinase Jnk3 antibody
Anti-C-Jun N-Terminal Kinase 3 antibody
Anti-MAPK10 antibody
Anti-JNK3 antibody
Anti-JNK3A antibody
Anti-PRKM10 antibody
Anti-SAPK1B antibody
Anti-Mitogen-Activated Protein Kinase 8 antibody
Anti-Map Kinase 8 antibody
Anti-Mapk 8 antibody
Anti-Jnk-46 antibody
Anti-Stress-Activated Protein Kinase 1c antibody
Anti-Sapk1c antibody
Anti-Stress-Activated Protein Kinase Jnk1 antibody
Anti-C-Jun N-Terminal Kinase 1 antibody
Anti-MAPK8 antibody
Anti-JNK1 antibody
Anti-PRKM8 antibody
Anti-SAPK1 antibody
Anti-SAPK1C antibody
Anti-Mitogen-Activated Protein Kinase 9 antibody
Anti-Map Kinase 9 antibody
Anti-Mapk 9 antibody
Anti-Jnk-55 antibody
Anti-Stress-Activated Protein Kinase 1a antibody
Anti-Sapk1a antibody
Anti-Stress-Activated Protein Kinase Jnk2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance