• Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-JAK3-Tyr980/981 antibody (STJ196341)

SKU:
STJ196341

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein kinase JAK3-Tyr980/981 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JAK3
Gene ID: 3718
Uniprot ID: JAK3_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse Jak3 (phospho-Tyr980 or 981)
Immunogen: Synthesized phosho peptide around human Jak3 (Tyr980 and 981)
Post Translational Modifications Tyrosine phosphorylated in response to IL-2 and IL-4. Dephosphorylation of Tyr-980 and Tyr-981 by PTPN2 negatively regulates cytokine-mediated signaling (Probable).
Function Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A and STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
Protein Name Tyrosine-Protein Kinase Jak3
Janus Kinase 3
Jak-3
Leukocyte Janus Kinase
L-Jak
Database Links Reactome: R-HSA-1266695
Reactome: R-HSA-201556
Reactome: R-HSA-5673001
Reactome: R-HSA-6785807
Reactome: R-HSA-8854691
Reactome: R-HSA-8983432
Reactome: R-HSA-8985947
Reactome: R-HSA-9020558
Reactome: R-HSA-9020958
Reactome: R-HSA-912526
Reactome: R-HSA-9679191
Cellular Localisation Endomembrane System
Peripheral Membrane Protein
Cytoplasm
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Jak3 antibody
Anti-Janus Kinase 3 antibody
Anti-Jak-3 antibody
Anti-Leukocyte Janus Kinase antibody
Anti-L-Jak antibody
Anti-JAK3 antibody

Information sourced from Uniprot.org

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