| Host: | Rabbit |
| Applications: | WB/ELISA/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein kinase JAK2 and Tyrosine-protein kinase JAK3-Tyr966/939 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC-P 1:50-300 ELISA 2000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | JAK2 JAK3 |
| Gene ID: | 3717 3718 |
| Uniprot ID: | JAK2_HUMAN JAK3_HUMAN |
| Specificity: | This antibody detects endogenous levels of JAK2/3 (Phospho-Tyr966/939) Antibody at Human:Y966/939;Mouse:Y966/935;Rat:Y966/935, It doesn t reacte with total protein. |
| Immunogen: | Synthesized Phospho peptide derived from the human JAK2/3.at the amino acid range T966 |
| Post Translational Modifications | Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity. Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition. Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation. |
| Function | Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation. Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. |
| Protein Name | Androgen Receptor Dihydrotestosterone Receptor Nuclear Receptor Subfamily 3 Group C Member 4 |
| Database Links | Reactome: R-HSA-3371497 Reactome: R-HSA-383280 Reactome: R-HSA-4090294 Reactome: R-HSA-5625886 Reactome: R-HSA-5689880 Reactome: R-HSA-8940973 |
| Cellular Localisation | Nucleus Cytoplasm Detected At The Promoter Of Target Genes Predominantly Cytoplasmic In Unligated Form But Translocates To The Nucleus Upon Ligand-Binding Can Also Translocate To The Nucleus In Unligated Form In The Presence Of Rack1 |
| Alternative Antibody Names | Anti-Androgen Receptor antibody Anti-Dihydrotestosterone Receptor antibody Anti-Nuclear Receptor Subfamily 3 Group C Member 4 antibody Anti-AR antibody Anti-DHTR antibody Anti-NR3C4 antibody |
Information sourced from Uniprot.org