Anti-Phospho-ITPR1-Ser1756 antibody (STJ196331)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phosho peptide around human IP3 Receptor (Ser1756)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Inositol 1, 4, 5-trisphosphate receptor type 1-Ser1756 for use in WB and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ITPR1 |
| Gene ID | 3708 |
| Uniprot ID | ITPR1_HUMAN |
| Immunogen | Synthesized phosho peptide around human IP3 Receptor (Ser1756) |
| Specificity | This antibody detects endogenous levels of Human Mouse Rat IP3 Receptor (phospho-Ser1756) |
Additional Info
| Post Translational Modifications | Polyubiquitinated. Polyubiquitination targets ITPR1 for proteasomal degradation. Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked. Phosphorylated by cAMP kinase (PKA) enhances calcium release. Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1. Phosphorylated on tyrosine residues. Palmitoylated by ZDHHC6 in immune cells, leading to regulation of ITPR1 stability and function. |
| Function | Inositol 1,4,5-trisphosphate-gated calcium channel that, upon inositol 1,4,5-trisphosphate binding, mediates calcium release from the endoplasmic reticulum (ER). Undergoes conformational changes upon ligand binding, suggesting structural flexibility that allows the channel to switch from a closed state, capable of interacting with its ligands such as 1,4,5-trisphosphate and calcium, to an open state, capable of transferring calcium ions across the ER membrane. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CAMK2 complex. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1. Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix. Regulates fertilization and egg activation by tuning the frequency and amplitude of calcium oscillations. |
| Protein Name | Inositol 1 -4 -5-Trisphosphate-Gated Calcium Channel Itpr1Ip3 Receptor Isoform 1Ip3r 1Insp3r1Inositol 1 -4 -5 Trisphosphate ReceptorInositol 1 -4 -5-Trisphosphate Receptor Type 1Type 1 Inositol 1 -4 -5-Trisphosphate ReceptorType 1 Insp3 Receptor |
| Database Links | Reactome: R-HSA-112043Reactome: R-HSA-114508Reactome: R-HSA-139853Reactome: R-HSA-1489509Reactome: R-HSA-2029485Reactome: R-HSA-2871809Reactome: R-HSA-381676Reactome: R-HSA-4086398Reactome: R-HSA-418457Reactome: R-HSA-422356Reactome: R-HSA-5218921Reactome: R-HSA-5578775Reactome: R-HSA-5607763Reactome: R-HSA-9664323Reactome: R-HSA-983695 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinCytoplasmic VesicleSecretory Vesicle MembraneCytoplasmPerinuclear RegionFound In A Complex With Hspa9 And Vdac1 At The Endoplasmic Reticulum-Mitochondria Contact Sites |
| Alternative Antibody Names | Anti-Inositol 1 -4 -5-Trisphosphate-Gated Calcium Channel Itpr1 antibodyAnti-Ip3 Receptor Isoform 1 antibodyAnti-Ip3r 1 antibodyAnti-Insp3r1 antibodyAnti-Inositol 1 -4 -5 Trisphosphate Receptor antibodyAnti-Inositol 1 -4 -5-Trisphosphate Receptor Type 1 antibodyAnti-Type 1 Inositol 1 -4 -5-Trisphosphate Receptor antibodyAnti-Type 1 Insp3 Receptor antibodyAnti-ITPR1 antibodyAnti-INSP3R1 antibody |
Information sourced from Uniprot.org