• Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using CD18/ITGB2 (Phospho-Thr758) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ITGB2-Thr758 antibody (720-769 aa) (STJ91331)

SKU:
STJ91331

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Integrin beta-2-Thr758 (720-769 aa) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGB2
Gene ID: 3689
Uniprot ID: ITB2_HUMAN
Immunogen Region: 720-769 aa
Specificity: Phospho-Integrin Beta 2 (T758) Polyclonal Antibody detects endogenous levels of Integrin Beta 2 protein only when phosphorylated at T758.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CD18/ITGB2 around the phosphorylation site of Thr758 at the amino acid range 720-769
Post Translational Modifications Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
Function Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15.the interaction is mediated by ITGAL. Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages. In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils.
Protein Name Integrin Beta-2
Cell Surface Adhesion Glycoproteins Lfa-1/Cr3/P150 -95 Subunit Beta
Complement Receptor C3 Subunit Beta
Cd Antigen Cd18
Database Links Reactome: R-HSA-166016
Reactome: R-HSA-198933
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-6785807
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Alternative Antibody Names Anti-Integrin Beta-2 antibody
Anti-Cell Surface Adhesion Glycoproteins Lfa-1/Cr3/P150 -95 Subunit Beta antibody
Anti-Complement Receptor C3 Subunit Beta antibody
Anti-Cd Antigen Cd18 antibody
Anti-ITGB2 antibody
Anti-CD18 antibody
Anti-MFI7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance