• Western blot analysis of lysates from 1) Hela, 2) 3T3, 3) Jurkat cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • Western blot analysis of lysates from HeLa cells treated with UV 15', using Integrin beta1 (Phospho-Thr788) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Integrin Beta 1 (T788) Polyclonal Antibody
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Integrin beta1 (Phospho-Thr788) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from 1) Hela, 2) 3T3, 3) Jurkat cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-ITGB1-Thr788 antibody (749-798 aa) (STJ90303)

SKU:
STJ90303

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Integrin beta-1-Thr788 (749-798 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGB1
Gene ID: 3688
Uniprot ID: ITB1_HUMAN
Immunogen Region: 749-798 aa
Specificity: Phospho-Integrin Beta 1 (T788) Polyclonal Antibody detects endogenous levels of Integrin Beta 1 protein only when phosphorylated at T788.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Integrin beta1 around the phosphorylation site of Thr788 at the amino acid range 749-798
Function Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 acts as a receptor for fibronectin FN1 and mediates R-G-D-dependent cell adhesion to FN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling. Plays an important role in myoblast differentiation and fusion during skeletal myogenesis. Isoform 2: Interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). Isoform 5: Isoform 5 displaces isoform 1 in striated muscles. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8. (Microbial infection) Acts as a receptor for Cytomegalovirus/HHV-5. (Microbial infection) Acts as a receptor for Epstein-Barr virus/HHV-4. (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human parvovirus B19. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus. (Microbial infection) Acts as a receptor for Mammalian reovirus. (Microbial infection) In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. (Microbial infection) Interacts with CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi. Integrin ITGA3:ITGB1 may act as a receptor for R.delemar CotH7 in alveolar epithelial cells, which may be an early step in pulmonary mucormycosis disease progression. (Microbial infection) May serve as a receptor for adhesin A (nadA) of N.meningitidis.
Protein Name Integrin Beta-1
Fibronectin Receptor Subunit Beta
Glycoprotein Iia
Gpiia
Vla-4 Subunit Beta
Cd Antigen Cd29
Database Links Reactome: R-HSA-1566948
Reactome: R-HSA-1566977
Reactome: R-HSA-198933
Reactome: R-HSA-202733
Reactome: R-HSA-210991
Reactome: R-HSA-2129379
Reactome: R-HSA-216083
Reactome: R-HSA-2173789
Reactome: R-HSA-3000157
Reactome: R-HSA-3000170
Reactome: R-HSA-3000178
Reactome: R-HSA-416700
Reactome: R-HSA-445144
Reactome: R-HSA-446343
Reactome: R-HSA-447041
Reactome: R-HSA-5663220
Reactome: R-HSA-6785807
Reactome: R-HSA-75892
Reactome: R-HSA-8874081
Reactome: R-HSA-8875513
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013408
Reactome: R-HSA-9013423
Reactome: R-HSA-9609690
Reactome: R-HSA-9634597
Reactome: R-HSA-9679191
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Invadopodium Membrane
Ruffle Membrane
Recycling Endosome
Melanosome
Cleavage Furrow
Lamellipodium
Cell Junction
Focal Adhesion
Highly Enriched In Stage I Melanosomes
Located On Plasma Membrane Of Neuroblastoma Nmb7 Cells
In A Lung Cancer Cell Line
In Prometaphase And Metaphase
Localizes Diffusely At The Membrane And In A Few Intracellular Vesicles
In Early Telophase
Detected Mainly On The Matrix-Facing Side Of The Cells
By Mid-Telophase
Concentrated To The Ingressing Cleavage Furrow
Mainly To The Basal Side Of The Furrow
In Late Telophase
Concentrated To The Extending Protrusions Formed At The Opposite Ends Of The Spreading Daughter Cells
In Vesicles At The Base Of The Lamellipodia Formed By The Separating Daughter Cells
Colocalizes With Itgb1bp1 And Metastatic Suppressor Protein Nme2 At The Edge Or Peripheral Ruffles And Lamellipodia During The Early Stages Of Cell Spreading On Fibronectin Or Collagen
Translocates From Peripheral Focal Adhesions Sites To Fibrillar Adhesions In A Itgb1bp1-Dependent Manner
Enriched Preferentially At Invadopodia
Cell Membrane Protrusions That Correspond To Sites Of Cell Invasion
In A Collagen-Dependent Manner
Localized At Plasma And Ruffle Membranes In A Collagen-Independent Manner
Isoform 2: Does Not Localize To Focal Adhesions
Isoform 5: Cell Membrane
Sarcolemma
In Cardiac Muscle
Isoform 5 Is Found In Costameres And Intercalated Disks
Alternative Antibody Names Anti-Integrin Beta-1 antibody
Anti-Fibronectin Receptor Subunit Beta antibody
Anti-Glycoprotein Iia antibody
Anti-Gpiia antibody
Anti-Vla-4 Subunit Beta antibody
Anti-Cd Antigen Cd29 antibody
Anti-ITGB1 antibody
Anti-FNRB antibody
Anti-MDF2 antibody
Anti-MSK12 antibody

Information sourced from Uniprot.org

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