• Immunohistochemistry analysis of paraffin-embedded human brain, using IRAK1 (Phospho-Ser376) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-IRAK1-Ser376 antibody (342-391 aa) (STJ91205)

SKU:
STJ91205

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Interleukin-1 receptor-associated kinase 1-Ser376 (342-391 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IRAK1
Gene ID: 3654
Uniprot ID: IRAK1_HUMAN
Immunogen Region: 342-391 aa
Specificity: Phospho-IRAK-1 (S376) Polyclonal Antibody detects endogenous levels of IRAK-1 protein only when phosphorylated at S376.
Immunogen: The antiserum was produced against synthesized peptide derived from the human IRAK1 around the phosphorylation site of Ser376 at the amino acid range 342-391
Post Translational Modifications Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity. Polyubiquitinated by TRAF6 after cell stimulation with IL-1-beta by PELI1, PELI2 and PELI3. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Ubiquitination promotes interaction with NEMO/IKBKG. Also sumoylated.leading to nuclear translocation.
Function Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
Protein Name Interleukin-1 Receptor-Associated Kinase 1
Irak-1
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-166058
Reactome: R-HSA-168638
Reactome: R-HSA-209543 P51617-1
Reactome: R-HSA-209560
Reactome: R-HSA-445989
Reactome: R-HSA-450302
Reactome: R-HSA-450321
Reactome: R-HSA-6811558
Reactome: R-HSA-8986944
Reactome: R-HSA-9020702
Reactome: R-HSA-937039
Reactome: R-HSA-9705671
Reactome: R-HSA-975110
Reactome: R-HSA-975138
Reactome: R-HSA-975144
Reactome: R-HSA-975155
Reactome: R-HSA-975871
Cellular Localisation Cytoplasm
Nucleus
Lipid Droplet
Translocates To The Nucleus When Sumoylated
Rsad2/Viperin Recruits It To The Lipid Droplet
Alternative Antibody Names Anti-Interleukin-1 Receptor-Associated Kinase 1 antibody
Anti-Irak-1 antibody
Anti-IRAK1 antibody
Anti-IRAK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance