Anti-Phospho-Integrin Beta 4-Tyr-1526 antibody (STJA0041256)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPhospho-Integrin Beta 4 (Tyr-1526) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1526 of human Integrin Beta 4. This peptide sequence is found in all three Integrin Beta 4 isoforms and is highly conserved in
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Integrin Beta 4-Tyr-1526 for use in ELISA, WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000ICC 1:100 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20ยฐC is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20ยฐC. |
Target Information
| Gene Symbol | ITGB4 |
| Gene ID | 3691 |
| Uniprot ID | ITB4_HUMAN |
| Immunogen | Phospho-Integrin Beta 4 (Tyr-1526) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1526 of human Integrin Beta 4. This peptide sequence is found in all three Integrin Beta 4 isoforms and is highly conserved in |
| Immunogen Sequence | Human |
| Specificity | This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to unphosphorylated Integrin Beta 4 (Tyr-1526) peptide before affinity purification using phospho-Integrin Beta 4 (Tyr-1526) peptide (without carrier). The purified antibody |
Additional Info
| Tissue Specificity | Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. |
| Post Translational Modifications | Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secondary association with tertaspanins. |
| Function | Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling. |
| Protein Name | Integrin Beta-4Gp150Cd Antigen Cd104 |
| Database Links | Reactome: R-HSA-2022090Reactome: R-HSA-3000157Reactome: R-HSA-3000170Reactome: R-HSA-446107Reactome: R-HSA-9725554 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinLipid-AnchorCell JunctionHemidesmosomeColocalizes With Dst At The Leading Edge Of Migrating Keratinocytes |
| Alternative Antibody Names | Anti-Integrin Beta-4 antibodyAnti-Gp150 antibodyAnti-Cd Antigen Cd104 antibodyAnti-ITGB4 antibody |
Information sourced from Uniprot.org