Anti-Phospho-IGFBP3-Ser183 antibody (151-200 aa) (STJ91161)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human IGFBP-3 around the phosphorylation site of Ser183 at the amino acid range 151-200
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Insulin-like growth factor-binding protein 3-Ser183 (151-200 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IGFBP3 |
| Gene ID | 3486 |
| Uniprot ID | IBP3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human IGFBP-3 around the phosphorylation site of Ser183 at the amino acid range 151-200 |
| Immunogen Region | 151-200 aa |
| Specificity | Phospho-IGFBP3 (S183) Polyclonal Antibody detects endogenous levels of IGFBP3 protein only when phosphorylated at S183. |
Additional Info
| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. Phosphorylated by CK2.resulting in decreased nuclear localization. |
| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis. Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1. Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade. In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 3Ibp-3Igf-Binding Protein 3Igfbp-3 |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-6803211Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedNucleus |
| Alternative Antibody Names | Anti-Insulin-Like Growth Factor-Binding Protein 3 antibodyAnti-Ibp-3 antibodyAnti-Igf-Binding Protein 3 antibodyAnti-Igfbp-3 antibodyAnti-IGFBP3 antibodyAnti-IBP3 antibody |
Information sourced from Uniprot.org