• Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night
  • Immunohistochemistry analysis of paraffin-embedded human brain, using IGFBP-3 (Phospho-Ser183) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-IGFBP3-Ser183 antibody (151-200 aa) (STJ91161)

SKU:
STJ91161

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Insulin-like growth factor-binding protein 3-Ser183 (151-200 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGFBP3
Gene ID: 3486
Uniprot ID: IBP3_HUMAN
Immunogen Region: 151-200 aa
Specificity: Phospho-IGFBP3 (S183) Polyclonal Antibody detects endogenous levels of IGFBP3 protein only when phosphorylated at S183.
Immunogen: The antiserum was produced against synthesized peptide derived from the human IGFBP-3 around the phosphorylation site of Ser183 at the amino acid range 151-200
Post Translational Modifications Phosphorylated by FAM20C in the extracellular medium. Phosphorylated by CK2.resulting in decreased nuclear localization.
Function Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis. Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1. Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade. In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis.
Protein Name Insulin-Like Growth Factor-Binding Protein 3
Ibp-3
Igf-Binding Protein 3
Igfbp-3
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-6803211
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Nucleus
Alternative Antibody Names Anti-Insulin-Like Growth Factor-Binding Protein 3 antibody
Anti-Ibp-3 antibody
Anti-Igf-Binding Protein 3 antibody
Anti-Igfbp-3 antibody
Anti-IGFBP3 antibody
Anti-IBP3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance