• Western blot of immunoprecipitates from HEK 293 cells transfected with 1) Mock, 2) IFNAR1 WT, and 3) IFNAR1 S535A and S539A mutants. Specific immunolabeling of the ~110 kDa to ~130 kDa IFNAR1 WT (2) is shown in the first blot, as the immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (3). The specific immunolabeling is blocked by the phosphopeptide (+) used as the antigen in the second blot.

Anti-Phospho-IFNAR1-Ser535/539 antibody (STJA0003673)

SKU:
STJA0003673-100

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Host: Rabbit
Applications: WB
Reactivity: Human/Bovine/Canine/Non-Human Primates/Mouse/Rat/Sheep
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-IFNAR1-Ser535/539 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFNAR1
Gene ID: 3454
Uniprot ID: INAR1_HUMAN
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 conjugated to KLH.
Tissue Specificity IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.
Post Translational Modifications Ubiquitinated, leading to its internalization and degradation. Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation. The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex. Phosphorylated on tyrosine residues in response to interferon-binding: phosphorylation by TYK2 tyrosine kinase creates docking sites for STAT proteins. Phosphorylated on serine residues in response to interferon binding.this promotes interaction with FBXW11 and ubiquitination. Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2.
Function Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response. Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another. The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors. STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes. Can also act independently of IFNAR2: form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway.
Protein Name Interferon Alpha/Beta Receptor 1
Ifn-R-1
Ifn-Alpha/Beta Receptor 1
Cytokine Receptor Class-Ii Member 1
Cytokine Receptor Family 2 Member 1
Crf2-1
Type I Interferon Receptor 1
Database Links Reactome: R-HSA-909733
Reactome: R-HSA-912694
Reactome: R-HSA-9679191
Reactome: R-HSA-9705671
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Late Endosome
Lysosome
Interferon Binding Triggers Internalization Of The Receptor From The Cell Membrane Into Endosomes And Then Into Lysosomes
Alternative Antibody Names Anti-Interferon Alpha/Beta Receptor 1 antibody
Anti-Ifn-R-1 antibody
Anti-Ifn-Alpha/Beta Receptor 1 antibody
Anti-Cytokine Receptor Class-Ii Member 1 antibody
Anti-Cytokine Receptor Family 2 Member 1 antibody
Anti-Crf2-1 antibody
Anti-Type I Interferon Receptor 1 antibody
Anti-IFNAR1 antibody
Anti-IFNAR antibody

Information sourced from Uniprot.org

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