• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using hnRPD (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from HUVEC and 293 cells, using hnRPD (Phospho-Ser83) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using hnRPD (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HNRNPD-Ser83 antibody (49-98 aa) (STJ91094)

SKU:
STJ91094

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Heterogeneous nuclear ribonucleoprotein D0-Ser83 (49-98 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPD
Gene ID: 3184
Uniprot ID: HNRPD_HUMAN
Immunogen Region: 49-98 aa
Specificity: Phospho-hnRNP D0 (S83) Polyclonal Antibody detects endogenous levels of hnRNP D0 protein only when phosphorylated at S83.
Immunogen: The antiserum was produced against synthesized peptide derived from the human hnRPD around the phosphorylation site of Ser83 at the amino acid range 49-98
Function Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation.
Protein Name Heterogeneous Nuclear Ribonucleoprotein D0
Hnrnp D0
Au-Rich Element Rna-Binding Protein 1
Database Links Reactome: R-HSA-450408
Reactome: R-HSA-72163
Reactome: R-HSA-72203
Cellular Localisation Nucleus
Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Component Of Ribonucleosomes
Cytoplasmic Localization Oscillates Diurnally
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoprotein D0 antibody
Anti-Hnrnp D0 antibody
Anti-Au-Rich Element Rna-Binding Protein 1 antibody
Anti-HNRNPD antibody
Anti-AUF1 antibody
Anti-HNRPD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance