• Immunofluorescence analysis of COS7 cells, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-HMGN1-Ser21 antibody (10-59 aa) (STJ91313)

SKU:
STJ91313

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Non-histone chromosomal protein HMG-14-Ser21 (10-59 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMGN1
Gene ID: 3150
Uniprot ID: HMGN1_HUMAN
Immunogen Region: 10-59 aa
Specificity: Phospho-HMG-14 (S21) Polyclonal Antibody detects endogenous levels of HMG-14 protein only when phosphorylated at S21.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HMG14 around the phosphorylation site of Ser21 at the amino acid range 10-59
Function Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
Protein Name Non-Histone Chromosomal Protein Hmg-14
High Mobility Group Nucleosome-Binding Domain-Containing Protein 1
Database Links Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Cellular Localisation Nucleus
Cytoplasm
Cytoplasmic Enrichment Upon Phosphorylation
The Rna Edited Version Localizes To The Nucleus
Alternative Antibody Names Anti-Non-Histone Chromosomal Protein Hmg-14 antibody
Anti-High Mobility Group Nucleosome-Binding Domain-Containing Protein 1 antibody
Anti-HMGN1 antibody
Anti-HMG14 antibody

Information sourced from Uniprot.org

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