• Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T45 antibody (STJ11101034) at 1:1000 dilution. Hela cells, NIH/3T3 cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-T45 Rabbit polyclonal antibody (STJ11101034) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human tonsil using Phospho-Histone H3-T45 antibody (STJ11101034) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-T45 Rabbit polyclonal antibody (STJ11101034) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat liver using Phospho-Histone H3-T45 antibody (STJ11101034) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using Phospho-Histone H3-T45 Rabbit polyclonal antibody (STJ11101034) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H3-T45 antibody (STJ11101034) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-Histone H3-T45 in paraffin-embedded mouse testis using Phospho-Histone H3-T45 Rabbit polyclonal antibody (STJ11101034) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using Phospho-Histone H3-T45 rabbit polyclonal antibody (STJ11101034) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Phospho-Histone H3-T45 in paraffin-embedded rat liver using Phospho-Histone H3-T45 Rabbit polyclonal antibody (STJ11101034) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-T45 rabbit polyclonal antibody (STJ11101034) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Phospho-Histone H3-T45 in paraffin-embedded human tonsil using Phospho-Histone H3-T45 Rabbit polyclonal antibody (STJ11101034) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-T45 rabbit polyclonal antibody (STJ11101034) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Phospho-Histone H3-T45 Rabbit polyclonal antibody (STJ11101034) at 1:1000 dilution. Hela cells, NIH/3T3 cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-Histone H3-T45 antibody (STJ11101034)

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STJ11101034

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Histone H3-T45 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around T45 of human Histone H3 (NP_003520.1).
Immunogen Sequence: PGTVA
Background This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER) , the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1 (S) , which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1 (U) , is constitutively expressed, and thought to function as a negative feedback regulator of XBP1 (S) , which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

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