| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Protein HIRA-Thr555 (521-570 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HIRA |
| Gene ID: | 7290 |
| Uniprot ID: | HIRA_HUMAN |
| Immunogen Region: | 521-570 aa |
| Specificity: | Phospho-HIRA (T555) Polyclonal Antibody detects endogenous levels of HIRA protein only when phosphorylated at T555. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human HIRA around the phosphorylation site of Thr555 at the amino acid range 521-570 |
| Post Translational Modifications | Sumoylated. Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo. |
| Function | Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. |
| Protein Name | Protein HiraTup1-Like Enhancer Of Split Protein 1 |
| Database Links | Reactome: R-HSA-2559584Reactome: R-HSA-9821993 |
| Cellular Localisation | NucleusPml BodyPrimarilyThough Not ExclusivelyLocalized To The NucleusLocalizes To Pml Bodies Immediately Prior To Onset Of Senescence |
| Alternative Antibody Names | Anti-Protein Hira antibodyAnti-Tup1-Like Enhancer Of Split Protein 1 antibodyAnti-HIRA antibodyAnti-DGCR1 antibodyAnti-HIR antibodyAnti-TUPLE1 antibody |
Information sourced from Uniprot.org