| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Homeodomain-interacting protein kinase 1-Tyr352/361/359 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HIPK1 |
| Gene ID: |
204851 |
| Uniprot ID: |
HIPK1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of HIPK1/2/3 (Phospho-Tyr352/361/359) at Human, Mouse, Rat |
| Immunogen: |
Synthesized peptide derived from human HIPK1/2/3 (Phospho-Tyr352/361/359) |
| Tissue Specificity | Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. Isoform 2 is highly expressed in testis. Expressed in both androgen-dependent and androgen-independent prostate cancer cells. |
| Post Translational Modifications | Autophosphorylated. Phosphorylated and activated by JNK1. Degraded by PARK7 at the protein level. Sumoylated. When conjugated it is directed to nuclear speckles. SENP1-mediated desumoylation is mediated by TNF in response to stress stimuli, triggering transient translocation from nucleus to cytoplasm. |
| Function | Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation. Phosphorylates PAGE4 at 'Thr-51' which is critical for the ability of PAGE4 to potentiate the transcriptional activator activity of JUN. |
| Protein Name | Homeodomain-Interacting Protein Kinase 1Nuclear Body-Associated Kinase 2 |
| Database Links | Reactome: R-HSA-2032785Reactome: R-HSA-5578768Reactome: R-HSA-6804756 |
| Cellular Localisation | NucleusCytoplasmNucleus SpecklePredominantly NuclearTranslocates From Nucleus To Cytoplasm In Response To Stress Stimuli Via Senp1-Mediated Desumoylation |
| Alternative Antibody Names | Anti-Homeodomain-Interacting Protein Kinase 1 antibodyAnti-Nuclear Body-Associated Kinase 2 antibodyAnti-HIPK1 antibodyAnti-KIAA0630 antibodyAnti-MYAK antibodyAnti-NBAK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
